The Diagnostic Network Optimisation Sub-community of Practice (DNO Sub-CoP) is a dedicated segment of ASLM’s LabCoP. The DNO Sub-CoP is a collaboration of ASLM and FIND, funded by the Bill & Melinda Gates Foundation. The DNO Sub-CoP gathers country teams (made up of laboratorians, clinicians, and representatives from ministries of health who support DNO activities in their country) and stakeholders (implementing partners, regulatory and technical agencies) who share challenges, solutions and best practices for optimising their diagnostic network.