Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

madhukar.pai · December 6, 2020, 2:11am

Highlights

CRISPR-Cas13a can quantitatively detect SARS-CoV-2 RNA without pre-amplification

Combining crRNAs targeting multiple regions of the viral RNA enhances sensitivity

Cas13a can accurately and rapidly quantify SARS-CoV-2 RNA in patient samples

A mobile phone-based device allows for portable and sensitive read-out of the assay

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